Background and Significance of Study
The Delta Smelt (Hypomesus transpacificus) and Longfin Smelt (Spirinchus thaleichthys) are two threatened osmerid species native to the San Francisco Estuary (SFE). They have experienced steep declines since the 1980s, most notably attributed to habitat modifications, entrainment in water divisions, and changes in the estuary’s biota. Both species are subject to intensive monitoring efforts that rely on morphological identifications. However, these identifications can be challenging due to damage and decomposition of fish caught in traps as well as the co-occurrence of a closely related introduced fish, the Wakasagi (Hypomesus nipponensis). Field differentiation of Delta Smelt and Wakasagi is particularly difficult because of their morphological similarity during early life stages and their capacity for hybridization.
We are seeking to improve current species identification methods by using restriction site-associated DNA sequencing (RAD-seq) to develop a SNP marker panel for identifying Delta Smelt, Longfin Smelt, Wakasagi, and their hybrid progeny. We will use this SNP panel on a group of osmerids collected in the Yolo Bypass (where Wakasagi have been detected with increasing frequency) to detect hybrids and quantify the accuracy of morphological species identifications. Our goal is that this SNP panel will constitute an accurate, efficient, and cost-effective resource to complement traditional survey methods in the SFE.